The actual NSCA Training Standards can be found here: Link Removed (Cover letter and three pdf pages.)
There is NO requirement to shoot one handed or opposite handed. However, individual instructors may require it.
Must must shoot 30 rounds (25 rounds for a five shot revolver.) 70% must be scored on the B26 or B21 humanoid target to pass.
Of those 30 rounds, six must be fired at three yards, twelve must be fired at five yards, and twelve must be fired at seven yards. (For five shot revolvers, 25 rounds, five fired at three yards, 10 at five yards, and 10 at seven yards.)
NOTE: The NSCA indicated plans to revise/update the NSCA Training Standards document; I would expect it by the summer of 2010.